Premium
THE EFFECT OF A CELL‐PERMEABLE DIACYLGLYCEROL ANALOGUE ON SINGLE Ca 2+ (Ba 2+ ) CHANNEL CURRENTS IN THE INSULIN‐SECRETING CELL LINE RINm5F
Author(s) -
Velasco J. M.,
Petersen O. H.
Publication year - 1989
Publication title -
quarterly journal of experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0144-8757
DOI - 10.1113/expphysiol.1989.sp003280
Subject(s) - depolarization , diacylglycerol kinase , biophysics , stimulation , protein kinase c , membrane potential , chemistry , biochemistry , biology , endocrinology , kinase
Depolarization evokes opening of single Ca 2+ (Ba 2+ ) channels of the ‘L’ type in the insulin‐secreting cell line RINm5F. Stimulation of the cells with the membrane‐permeable diacylglycerol analogue didecanoylglycerol (DC 10 ; 5 µg/ml) markedly enhanced the proportion of time the channels spent in the open state during depolarizing voltage pulses. Similar effects have previously been demonstrated with glyceraldehyde stimulation and it therefore seems possible that carbohydrate‐evoked cellular Ca 2+ uptake is mediated via protein kinase C activation.