Premium
THE EFFECT OF DINITROPHENOL ON MAGNESIUM TRANSPORT ACROSS AN ISOLATED PREPARATION OF SHEEP RUMEN EPITHELIUM
Author(s) -
Martens H.
Publication year - 1985
Publication title -
quarterly journal of experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0144-8757
DOI - 10.1113/expphysiol.1985.sp002943
Subject(s) - rumen , 2,4 dinitrophenol , magnesium , dinitrophenol , chemistry , epithelium , biochemistry , zoology , biology , fermentation , organic chemistry , genetics
Mg transport across an isolated preparation of sheep rumen epithelium was studied in vitro . The efflux (flux from the mucosal side to serosal side) and the influx (flux with serosal to mucosal side) were estimated with 28 Mg. Under physiological conditions a significant net efflux of Mg was observed, in which the unidirectional efflux was seven to eight times that of the influx. In experiments where ATP synthesis was blocked by the addition of dinitrophenol, Mg efflux was reduced to that of the influx. These observations support the conclusion that Mg absorption across the rumen wall of the sheep is an active process.