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THE EFFECT OF CHOLESTEROL OLEATE TREATMENT OF MICE ON THE ROSETTE FORMING CELL RESPONSE AGAINST SHEEP ERYTHROCYTES
Author(s) -
Smith I. I.,
Dewar A. Edna,
Ramage Elizabeth F.,
Stuart A. E.,
Adams W.
Publication year - 1976
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1976.sp002333
Subject(s) - mononuclear phagocyte system , spleen , phagocytosis , red pulp , immune system , lymph , cholesterol , antigen , endocrinology , biology , heterologous , medicine , immunology , chemistry , biochemistry , pathology , gene
Mice injected with a single dose of 60 mg cholesterol oleate emulsion showed substantial blockade of the mononuclear phagocyte system, measured by the rate of vascular clearance of radio‐labelled sheep erythrocytes. The labelled erythrocytes, in lipid treated mice, localized mainly in the spleen, contrasting with control mice in which localization was mainly in the liver. Treatment with this lipid, 24 hr before the intravenous injection of two different doses of sheep erythrocytes, resulted in significant depression of the rosette forming cell response in the spleen, whereas the responses in the lymph nodes of both control and lipid treated mice were at a low level and not significantly different. Intravenously administered cholesterol oleate emulsion is known to localize mainly in the Kupffer cells and in splenic red pulp macrophages. Cultured macrophages treated with this lipid show inhibition of antigen‐binding and depressed phagocytosis of heterologous erythrocytes. The lipid does not affect lymphocytes. These findings are in keeping with the hypothesis that macrophages play a direct role in the induction of an immune response against a particulate antigen.