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ACID HYDROLASES IN THE RABBIT IN HAEMORRHAGIC SHOCK
Author(s) -
Courtice F. C.,
Adams E. P.,
Shan A. D.,
Bishop D. M.
Publication year - 1974
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1974.sp002239
Subject(s) - acid phosphatase , lymphatic system , lymph , haemorrhagic shock , shock (circulatory) , chemistry , medicine , endocrinology , thoracic duct , biology , biochemistry , andrology , enzyme , pathology , immunology
Haemorrhagic shock was induced in rabbits by removal of 20 to 33 ml./kg of blood in up to 5 hr. Following haemorrhage there were on average 4 to 5‐fold increases in the levels of β‐glucuronidase, N‐acetyl‐β‐D‐glucosaminidase, acid ribonuclease and cathepsin D in the circulating plasma. Acid phosphatase levels fell at first, approximately parallel with the fall in the level of protein, and rose only when the animal was near death from haemorrhagic shock. Several experiments in which thoracic duct and hepatic lymph were collected showed that the lymphatic pathway was not essential for the increased levels of acid hydrolases in the plasma following haemorrhage. Biochemical and histochemical studies on the liver and proximal ileum indicated an increased permeability of the lysosomal membranes in these two regions. The results pointed to the liver as the likely source of origin of the increased circulating plasma levels of acid hydrolases in the rabbit in haemorrhagic shock.