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THE MITOSIS OF SHEEP BLOOD MONOCYTES IN TISSUE CULTURE
Author(s) -
Greenwood B.
Publication year - 1973
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1973.sp002230
Subject(s) - mitosis , monocyte , biology , microbiology and biotechnology , cell culture , tissue culture , macrophage , cell division , immunology , cell , andrology , medicine , genetics , in vitro
A technique is described by which sheep blood monocytes have been collected in tissue culture chambers and maintained in frequent changes of 100% autologous blood plasma. Such cells grow in size, and, having assumed a large macrophage form, they divide by mitosis. The cultures have been observed daily and their mitotic indices determined by counting 2,000 cells. These indices peak about the eighth day in culture. Mitotic activity then diminishes and usually dies out. The products of mitosis are two daughter cells which have macrophage size and morphology, and never at any time resemble fresh blood monocytes. These events have been confirmed by continuous recording with time‐lapse cine‐photomicrography. The work demonstrates the proliferative capacity of the mammalian blood monocyte. The cell collection technique and the observation and continuous recording of living cultures have been directed at refuting any possible criticism that mitoses were occurring in contaminating fibroblasts.