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TRANSIENT EFFECTS OF ETHACRYNIC ACID ON Na AND K MOVEMENTS IN CULTURED CELLS
Author(s) -
Lamb J. F.,
Lindsay R.
Publication year - 1973
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1973.sp002228
Subject(s) - incubation , chemistry , biophysics , diuretic , chloride , sodium , cell , membrane , transient (computer programming) , endocrinology , biochemistry , biology , organic chemistry , computer science , operating system
Ethacrynic acid produces a dose‐related inhibition of Na‐K transport across the cell membrane of L‐cells cultured as a monolayer. In addition, there is a transient increase in Na and K fluxes into and out of the cell. These effects are maximal at the shortest incubation period observed (30 sec) and gradually diminish until the fluxes at 20–30 min are not significantly different from the control values. There were no effects on chloride movements when cells were incubated in ethacrynate. The only likely explanation of these transient changes in cation flux would appear to be a temporary alteration in exchange diffusion of Na and K. The relationship of these changes to the diuretic effect of the drug is discussed.