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METABOLIC CHANGES INDUCED BY TISSUE INJURY: IN VITRO STUDIES WITH RAT LIVER SLICES
Author(s) -
Nitzan M.,
Groffman H.
Publication year - 1971
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1971.sp002104
Subject(s) - glycogen , liver injury , in vitro , liver tissue , alanine , endocrinology , medicine , biochemistry , chemistry , lipid metabolism , lactic acid , biology , amino acid , genetics , bacteria
To study the metabolic response to tissue injury, in vitro studies with rat liver slices have been conducted. Liver slices, from fasted rats which had undergone standard midline laparotomy 24 hr before, showed a 14‐fold increase in the incorporation of acetate‐2‐ 14 C into cholesterol, with no change in the conversion of the acetate to other lipid fractions or 14 CO 2 . The fate of L‐alanine‐ 14 C has also been determined. The same physical injury brought about a 7‐fold increase in the production of cholesterol‐ 14 C, a 66 per cent increase in the incorporation of the alanine into liver proteins, and a 35 per cent increase in the evolution of lactic acid‐ 14 C. The radioactivity of other lipid classes, 14 CO 2 , glucose or glycogen was not significantly different from the corresponding control values. These data are compatible with a striking and specific stimulating action of surgical injury upon cholesterogenesis. In addition, though the known effect of physical injury in the peripheral tissues is excessive breakdown of proteins, the results suggest enhanced hepatic protein synthesis after injury.