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INTRACELLULAR pH IN MATURE AND IMMATURE RABBIT RED CELLS
Author(s) -
Calvey T. N.
Publication year - 1970
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1970.sp002073
Subject(s) - intracellular , reticulocyte , intracellular ph , chemistry , chloride , biophysics , red blood cell , biochemistry , membrane , chromatography , biology , rna , organic chemistry , gene
The intracellular pH of rabbit red cells was determined from the distribution of (2‐ 14 C) DMO. Evidence is presented that the method provides a valid estimate of intracellular pH. In phosphate buffer at pH 7·40, the mean intracellular pH of the rabbit erythrocyte was 7·27. There was a close correspondence between the distribution ratios for hydrogen and chloride ions in normal rabbit blood. Values for intracellular pH, based on forty experiments in normal animals, were consistent with recent measurements of the membrane potential of mammalian red cells. The intracellular pH of the reticulocyte was deduced from the effect of acetylphenylhydrazide on the internal pH of rabbit red cells. At an external pH of 7·40, mean reticulocyte pH (7·32) was 0·06 pH units higher than mean erythrocyte pH (7·26). In preparations of reticulocytes, the distribution ratio for chloride ions was lower than the corresponding ratio for hydrogen ions. It is suggested that the presence in reticulocytes of small numbers of relatively alkaline mitochondria may be responsible for this phenomenon.