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ON THE PRESENCE OF GLUTAMINASE IN MUSCLE
Author(s) -
Ottaway J. H.
Publication year - 1969
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1969.sp002005
Subject(s) - glutaminase , skeletal muscle , medicine , endocrinology , cardiac muscle , kidney , mole , enzyme , biology , enzyme assay , specific activity , chemistry , biochemistry , glutamine , amino acid
The glutaminase activity of rat cardiac and skeletal muscle has been investigated, and compared with that of rat kidney. V max per g. of protein was 42 µmole/hr for heart muscle homogenates and 478 µmole/hr for kidney homogenates. Glutaminase activity was not detectable in about 50 per cent of the skeletal muscle homogenates; in the other 50 per cent the mean activity was about 30 µmole/g. protein/hr. The apparent K m of the enzyme in all 3 tissues was approximately 4 mM. Bovine growth hormone injected into rats for 5 days did not alter the level of glutaminase activity in kidney or cardiac muscle, but significantly increased the percentage of skeletal muscle homogenates in which glutaminase was detectable.