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CARBONIC ANHYDRASE ACTIVITY IN SALIVA IN MAN
Author(s) -
Szabo I.,
Mason D. K.,
Harden R. McG.
Publication year - 1966
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1966.sp001849
Subject(s) - saliva , carbonic anhydrase , enzyme assay , chemistry , enzyme , amylase , endocrinology , chromatography , medicine , biochemistry
The carbonic anhydrase activity has been measured in human parotid, submandibular and mixed saliva. In parotid saliva the activity was related to salivary flow rate, rising from less than 1·6 K/ml. at low flow rates to a maximum of about 2·2 K/ml. at higher rates of flow. The enzyme activity remained constant at flow rates greater than 0·8 ml./min. The concentration in submandibular saliva tended to be less than the concentration in parotid saliva. The enzyme activity in the blood was found to be 1·9 K/ml. Enzyme activity was not affected by storage at 0° C., 370° C. and room temperature for up to 48 hr. Similar values were found when saliva was collected under oil and when saliva was collected without oil.