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THE BEHAVIOUR OF MACROPHAGES LABELLED WITH COLLOIDAL CARBON DURING WOUND HEALING IN RABBIT EAR CHAMBERS
Author(s) -
Cliff W. J.
Publication year - 1966
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1966.sp001831
Subject(s) - granulation tissue , wound healing , pathology , trypan blue , lymphatic system , granulation , chemistry , macrophage , rabbit (cipher) , anatomy , biophysics , biology , medicine , materials science , cell , immunology , biochemistry , composite material , in vitro , statistics , mathematics
A method for injecting colloidal carbon into healing rabbit ear chambers has been described. By using this technique it was possible to label discrete populations of macrophages of the granulation tissue in five ear chambers in five rabbits. Observations on the cells so labelled confirmed that macrophages could be moved passively by currents in the fluid milieu present at the growing fringe of the granulation tissue. Once the labelled cells were incorporated into the fibrous scar tissue their movements were extremely slow, the highest rate recorded being 40 µ/day, and showed specificity for the perivascular regions which resulted in the characteristic distribution of macrophages along the blood vessels. The rate of this migration by the macrophages was inversely proportional to time and by 12 weeks after labelling was negligible. The carbon‐labelled macrophages were capable subsequently of concentrating intravenously injected trypan blue. The presence of lymphatic vessels had no significant influence on the removal of the carbon label.