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UPTAKE AND METABOLISM OF 14 C‐LABELLED TRIGLYCERIDE BY RETICULO‐ENDOTHELIAL CELLS
Author(s) -
Day A. J.,
Fidge N. H.,
GouldHurst P. R. S.,
Wahlqvist M. L.,
Wilkinson G. K.
Publication year - 1966
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1966.sp001819
Subject(s) - triglyceride , phospholipid , palmitic acid , fatty acid , chemistry , metabolism , hydrolysis , stearic acid , biochemistry , oleic acid , lymph , emulsion , cholesterol , chromatography , organic chemistry , membrane , psychology , psychiatry
The uptake and metabolism of 14 C‐labelled triglyceride by R.E. cells in rat lymph nodes has been investigated. After the intraperitoneal injection of a corn oil emulsion containing glyceryl tripalmitate‐1‐ 14 C uptake of labelled triglyceride by the R.E. cells in the nodes occurs. The uptake is accompanied by hydrolysis of the triglyceride and reincorporation of the liberated fatty acid mainly into phospholipid. Where cholesterol suspension is injected together with the triglyceride emulsion the 14 C‐labelled fatty acid is also incorporated into cholesterol ester in the nodes. Investigation by gas‐liquid chromatography of the labelled fatty acid in each of the lipid moieties in the nodes showed that some chain elongation of the 14 C‐labelled palmitic acid occurred, but that the stearic acid formed appeared only in the phospholipid fraction.