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CONTINUOUS CINEMATOGRAPHY OF HUMAN LYMPHOCYTES CULTURED WITH A PHYTOHÆMAGGLUTININ INCLUDING OBSERVATIONS ON CELL DIVISION AND INTERPHASE
Author(s) -
Roberts K. B.,
Marshall W. H.
Publication year - 1965
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1965.sp001803
Subject(s) - interphase , cell division , microbiology and biotechnology , division (mathematics) , motility , biology , incubation , cell , daughter , genetics , evolutionary biology , mathematics , biochemistry , arithmetic
Continuous time‐lapse cinematography of human small lymphocytes, in tissue culture with phytohæmagglutinin, has been carried out by confining the cells in microchambers. It has been shown that small lymphocytes can enlarge and divide after incubation in such cultures. The large cells so formed, and the daughter cells produced by their division, move in a characteristically lymphocytic fashion. The daughter cells have been shown to double their size and then themselves divide. Evidence is presented which suggests that a line of large proliferating cells with lymphocytic motility is formed and that these continue for some days to reproduce their own kind rather than differentiate into any other cell type. The time taken for cell division is about 30 min. Interphase has been measured on two occasions giving times of 23 and 38½ hr. respectively.