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THE GOLGI APPARATUS IN SMALL LYMPHOCYTES AND IN TRANSFORMED BLAST CELLS
Author(s) -
Coulson A. S.
Publication year - 1965
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1965.sp001792
Subject(s) - golgi apparatus , thiamine pyrophosphate , pyrophosphate , chemistry , mitosis , biochemistry , vesicle , enzyme , microbiology and biotechnology , membrane , cell , biology , cofactor
The Golgi apparatus can be demonstrated by incubating cells in a medium containing lead nitrate and either thiamine pyrophosphate or a nucleoside diphosphate. Enzyme activity at the Golgi membranes hydrolyses the thiamine pyrophosphate or nucleoside diphosphate and a precipitate of lead phosphate is formed. By converting this precipitate into lead sulphide the Golgi apparatus can be visualized microscopically. The enzyme activity in freshly separated human small lymphocytes is barely detectable. After tissue culture for 80‐90 hr. with an antigenic stimulus some of the small lymphocytes transform into blast cells. The transformed blast cells contain a well‐developed Golgi apparatus which is basically a figure‐of‐eight shape. Blast cells in mitosis do not contain a Golgi apparatus. This evidence suggests that the transformed blast cell is manufacturing protein, possibly antibody.