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METABOLISM OF CHOLESTEROL ESTER BY RETICULO‐ENDOTHELIAL CELLS
Author(s) -
Day A. J.,
Fidge N. H.,
Gould-Hurst P. R. S.,
Wilkinson G. K.
Publication year - 1965
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1965.sp001789
Subject(s) - lecithin , sphingomyelin , cholesterol , phospholipid , chemistry , metabolism , hydrolysis , lipid metabolism , intraperitoneal injection , biochemistry , endocrinology , biology , membrane
The metabolism of cholesterol ester by the reticulo‐endothelial cells in the sternal lymph nodes of rats was studied over a time period of 7 days. After the intraperitoneal injection of cholesterol‐ 3 H oleate‐l‐ 1 4C the uptake of ester by the RE cells in the nodes was shown to be accompanied by its partial hydrolysis. A progressive rise in the 3 H/ 14 C ratio of the node lipid extract showed that the turnover of the labelled oleate and its removal from the nodes was more rapid than that of the labelled cholesterol. In addition the fatty acid of the cholesterol ester was found to be incorporated into other lipid moieties in the nodes, particularly into phospholipid. Of this, lecithin and, to a lesser extent, sphingomyelin and cephalin were labelled.