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THE COMPOSITION OF THE PLASMA LIPOPROTEINS IN EXPERIMENTAL HYPERLIPAMIA INDUCED BY TRITON WR‐1339, CORTISONE, ALLOXAN AND HAEMORRHAGE IN RABBITS
Author(s) -
Courtice F. C.,
Munoz-Marcus M.
Publication year - 1964
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1964.sp001749
Subject(s) - cortisone , alloxan , chemistry , medicine , endocrinology , lipoprotein , plasma lipoprotein , composition (language) , ultracentrifuge , very low density lipoprotein , cholesterol , biochemistry , biology , diabetes mellitus , linguistics , philosophy
The lipoproteins in the plasma of rabbits in hyperlipoemic states iinduced by the administration of Triton WR‐1339, cortisone, alloxan or by repeated hbemorrhage have been separated in a preparative ultracentrifuge at densities of 1·019, 1·063 and 1·200 and the composition of each class determined by chemical analysis. In each of these conditions there was a considerable decrease in the level of the high‐density lipoproteins of d 1·063‐1·200 compared with normal or cholesterol‐fed animals. In Triton hyperlipæmia the β‐lipoproteins of d 1·019‐1·063 varied, but in cortisone, alloxan and hæmorrhage hyperlipæmias this class of lipoprotein practically disappeared. In these conditions, therefore, nearly all the lipoprotein was in the d 1·19 class. The mean percentages for the d {lt} 1·19 class for a group of ten Triton hyperlipæmic animals with a mean plasma total lipoprot, ein valuc of 3192 mg /100 ml. were PR 8·5, PL 25·3, TC 15·7 and TG 50·5. Corresponding values for a group of nine animals with hyperlipæmia induced by cortisone, alloxan or hæmorrhage and with a mean plasma total lipoprotein level of 1751 mg./100 ml. were PR 7·4, PL 14·9, TC 7·3 and TG 70·4. In each group the percentage of TG rose and the percentages of the other constituents fell with increasing degrees of lipoemia. These findings suggested that with increasing levels of TG the mean size of the complexes was increased. The administration of cortisone or Triton to cholesterol‐fed rabbits led to decrease in the levels of the d 1·063‐1·200 and d 1·019‐1·063 classes of lipoprotein and a considerable increase in the d < 1·019 class compared with control animals.