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THE GROWTH AND MITOSIS OF HUMAN SMALL LYMPHOCYTES AFTER INCUBATION WITH A PHYTOHÆMAGGLUTININ
Author(s) -
Marshall W. H.,
Roberts K. B.
Publication year - 1963
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1963.sp001645
Subject(s) - endoplasmic reticulum , mitosis , incubation , peripheral blood , suspension culture , suspension (topology) , lymphocyte , electron micrographs , biology , cell division , microbiology and biotechnology , cell , immunology , cell culture , chemistry , biochemistry , electron microscope , genetics , optics , physics , mathematics , homotopy , pure mathematics
Suspensions of human peripheral blood lymphocytes have been obtained by centrifuging blood in which the red cells were agglutinated with a phytohæmagglutinin (PHA). After 2 and 3 days culture, these suspensions frequently contained numerous large dividing cells. It is thought that it is the small lymphocyte which grows during the period of culture into a cell capable of dividing since on occasion greater than 98 per cent of the cells in the original suspension were small lymphocytes. The changed lymphocytes are pyroninophilic. Electron micrographs of these cells show no extensive endoplasmic reticulum.