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THE STRUCTURE OF CHYLOMICRA OBTAINED FROM THE THORACIC DUCT OF THE RAT
Author(s) -
Kay D.,
Robinson D. S.
Publication year - 1962
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1962.sp001605
Subject(s) - osmium tetroxide , electron microscope , anatomy , chemistry , materials science , biology , optics , physics
Chylomicra, obtained from the thoracic ducts of rats which have been fed olive oil, have been washed free from soluble lymph proteins, fixed in osmium tetroxide, and sectioned after embedding in Araldite. When they are adequately fixed, the chylomicra appear in the electron microscope to have circular crosssections, to be between 100 and 3500 mµ diameter and to have uniform electron density, except for a narrow peripheral band which stains more darkly. This band, which is believed to correspond to the site of the minor lipid and protein components of the chylomicra, is not a continuous structure but is made up of minute discrete specks.