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DEPOSITION OF PROTEIN IN DAMAGED LIVER CELLS
Author(s) -
Dixon Kendal C.
Publication year - 1962
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1962.sp001577
Subject(s) - intracellular , chemistry , deposition (geology) , protein aggregation , eosin , staining , carbon tetrachloride , lipid droplet , biophysics , biochemistry , biology , pathology , medicine , organic chemistry , paleontology , sediment
Globular masses of protein are formed in centrilobular cells of the livers of rats following injection of carbon tetrachloride. These aggregates are coloured by the oxidized tannin‐azo (OTA) method for protein as well as by the oxidized tannin‐oxazine (OTO) technique described in the preceding paper. The deposited material is anionophilic being stained by eosin and by erythrosin. Fat is also visible in the injured cells. The concomitant deposition of protein and lipid may be initiated by interference with the intracellular co‐dispersion of these substances in the micellar state. Aggregation into globules may then impede utilization and so promote the quantitative accumulation of both lipid and protein followed by further visible deposition. In this way protein and fat, which enter the injured cells from the blood or which are formed de novo inside the cells, may both accumulate so as to contribute further to these visible intracellular deposits. The formation of these aggregates of anionophilic protein largely accounts for the eosinophilia of damaged and necrotic liver cells.