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A COMPARISON OF THE OXIDATION OF CHOLESTEROL‐26‐ 14 C, PALMITATE‐1‐ 14 C AND TRIPALMITIN‐1‐ 14 C BY MACROPHAGES IN VITRO
Author(s) -
Day A. J.
Publication year - 1961
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1961.sp001557
Subject(s) - tripalmitin , cholesterol , chemistry , chylomicron , in vitro , in vivo , biochemistry , aqueous solution , lipoprotein , organic chemistry , biology , very low density lipoprotein , microbiology and biotechnology
The oxidation by macrophages of cholesterol‐26‐ 14 C to 14 CO 2 was compared with the oxidation by macrophages of 14 C‐labelled fatty acids (sodium palmitate‐1‐ 14 C) and 14 C‐labelled triglycerides (artificial emulsion of tripalmitin‐1‐ 14 C and chyle containing tripalmitin‐1‐ 14 C). Cholesterol was presented to rabbit macrophages in vitro as an aqueous solution (using Tween 20 to disperse the cholesterol), as an aqueous suspension and as cholesterol contained in chylomicra in rat chyle. It was shown that appreciable oxidation of 14 C‐labelled fatty acids and triglycerides could be brought about by macrophages but under similar circumstances no oxidation of the cholesterol side‐chain could be demonstrated in any of the preparations used. The failure of the macrophages in vitro to oxidize cholesterol probably reflects their behaviour in vivo with cholesterol taken up by macrophages in the arterial wall. The oxidation of other lipids by these cells and their failure to deal with cholesterol may then be associated with cholesterol deposition and with the pathogenesis of atheroma.