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THE ELIMINATION OF MOUSE AND BOVINE GLOBULINS FROM THE CIRCULATION OF NEWBORN AND ADULT MICE
Author(s) -
Pace M. Geraldine,
Dresser D. W.
Publication year - 1961
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1961.sp001555
Subject(s) - heterologous , gamma globulin , globulin , blood proteins , medicine , endocrinology , biology , chemistry , immunology , biochemistry , antibody , gene
The rate of elimination of 131 I labelled serum proteins from the circulation of mice has been calculated from estimations of radioactivity in the whole body and in samples of serum. In adult mice heterologous globulins are eliminated faster than homologous globulins, but in suckling mice no clear interpretation could be made of the results. It has also been shown that any one protein is eliminated faster by outbred mice than by CBA mice; this is certainly so in adults and possibly also in suckling mice. Estimations of the half‐lives of l31 I labelled proteins in suckling mice by counts of the radioactivity of the whole body, have been found to be inaccurate. This method gives longer half‐lives than counts of radioactivity of serum. This difference decreases with age so that in 3‐month‐old adult mice the half‐lives obtained by the two methods do not differ significantly. The unreliability of counts of the whole body may be due to the recirculation of the iodine label. A large amount of non‐protein‐bound radioactivity has been demonstrated in the gut and gut contents of sucklings, the amount of free label falling with increasing age. Counts of serum of sucklings show a slight decrease in the rate of elimination of 131 I labelled bovine gamma globulin with increasing age, although it has not been possible to determine if this is a real difference in the rate of elimination of the protein, or is an artefact due to a small amount of reincorporation of 131 I into the serum proteins of suckling mice.