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THE EFFECT OF TOLBUTAMIDE AND INSULIN ON THE REDUCED GLUTATHIONE CONTENT OF THE BLOOD AND LIVER OF NORMAL AND ALLOXAN‐DIABETIC RATS
Author(s) -
Gryglewski R.,
Duncan L. J. P.
Publication year - 1961
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1961.sp001527
Subject(s) - tolbutamide , glutathione , medicine , endocrinology , alloxan , chemistry , insulin , perfusion , pancreas , diabetes mellitus , biochemistry , enzyme
The blood reduced glutathione (GSH) concentration of normal rats was reduced by the intra‐cardiac injection of tolbutamide but not by insulin or sulphadiazine. Tolbutamide did not lower the blood GSH of severely alloxandiabetic rats. No significant change in the GSH content of the pancreas, kidney or liver was observed in normal rats following the injection of tolbutamide or sulphadiazine. The fall in the GSH content of liver homogenates incubated in vitro was accelerated by tolbutamide except when glucose was added to give a high concentration in the homogenate. Perfusion of the liver in situ with insulin or sulphadiazine did not alter its GSH content but perfusion with tolbutamide significantly reduced the GSH content. These findings are discussed in relation to possible mechanisms of the hypoglycaemic action of the sulphonylureas.