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THE EFFECT OF METABOLIC STIMULANTS ON THE OXYGEN UPTAKE OF NORMAL AND LEUKÆMIC HUMAN LEUCOCYTES IN VITRO
Author(s) -
Bisset Sheenah K.,
Alexander W. D.
Publication year - 1960
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1960.sp001438
Subject(s) - chronic myeloid leukaemia , chemistry , oxygen , in vitro , myeloid , myeloid cells , pharmacology , immunology , biochemistry , biology , cell , organic chemistry
The metabolic stimulants used were sodium salicylate (10–160 mg./100 ml. medium) and triiodothyroacetic acid (TRIAC) (10–50 µg./100 ml. medium). Leucocytes from three groups were studied: normal individuals, patients with chronic lymphatic leukæmia, and patients with chronic myeloid leukæmia. Determination of oxygen consumption was made by the direct method in Warburg respirometers at 37°C. There was no significant difference between the oxygen consumption of normal or of either type of leukæmic leucocytes in absence of a metabolic stimulant. In presence of sodium salicylate, the increase in oxygen consumption of normal leucocytes was significantly greater than that of either type of leukæmic leucocyte. Addition of triiodothyroacetic acid did not produce any significant increase in the oxygen consumption of normal or chronic lymphatic leukæmic leucocytes: it did, however, produce a significant increase in the oxygen consumption of chronic myeloid leukæmic leucocytes.