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AUTORADIOGRAPHIC OBSERVATIONS ON THE MUCOUS CELLS OF THE STOMACH AND INTESTINE
Author(s) -
Jennings M. A.,
Florey H. W.
Publication year - 1956
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1956.sp001171
Subject(s) - mucin , mucus , golgi apparatus , foveolar cell , stomach , duodenum , staining , small intestine , epithelium , secretion , intracellular , gastric chief cell , chemistry , histogenesis , pathology , biology , anatomy , cell , gastric mucosa , medicine , endocrinology , biochemistry , immunohistochemistry , ecology
1. Certain of the epithelial cells of the stomach and duodenum take up 35 S from intravenously injected Na 2 35 SO 4 and incorporate it in the mucous secretion. The particular types of cell which do this vary from species to species. 2. The goblet cells of the small and large intestines of all the species examined take up 35 S from Na 2 35 SO 4 . The 35 S iS first concentrated in the supranuclear or “Golgi body” region, before being distributed through the intracellular mucin. 3. The 35 S is taken up rapidly, and radioactive mucin is being discharged within an hour or so of the injection of Na 2 35 SO 4 . Most of the radioactivity has disappeared from the cells in 24 hours. 4. After the intravenous injection of [ 35 S] methionine, radioactivity appears diffusely in gastro‐intestinal epithelium, and shows no concentration in the mucus‐producing cells. 5. Some implications of the results are discussed. We wish to acknowledge our debt to Mr. J. Kent who assisted in all the animal work, Mr. D. Jerrome who prepared the autoradiographs and devised suitable staining methods, and Mr. B. H. Glass who took the photographs.