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THE CHEMICAL COMPOSITION OF CHYLOMICRA IN THE RAT
Author(s) -
Robinson D. S.
Publication year - 1955
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1955.sp001103
Subject(s) - chyle , phospholipid , lecithinase , chemistry , emulsion , biochemistry , chromatography , chylomicron , lipase , phosphatidylcholine , cholesterol , lipoprotein , enzyme , very low density lipoprotein , medicine , membrane , complication
1. It has been shown that lipid particles prepared from rat chyle, and free from soluble lymph proteins, contain neutral fat, phospholipid and cholesterol but no ether‐insoluble nitrogen. 2. Such a preparation resembles whole chyle in its state of dispersion, emulsion stability and resistance to immediate clearing by pancreatic lipase preparations. 3. The preincubation of such a preparation or of whole chyle with Clostridium welchii lecithinase destroys the emulsion stability and renders both preparations immediately susceptible to clearing by pancreatic lipase. 4. When the lipid particles recovered from rat chyle are incubated with normal plasma for 12 hours and subsequently recovered free from the soluble plasma proteins, the stability of the particle emulsion is destroyed. The preparation is immediately susceptible to clearing by pancreatic lipase. 5. On analysis, such preparations contain neutral fat, phospholipid and nitrogen. The phospholipid is not ether‐extractable and is present in smaller concentrations than before incubation with normal plasma. 6. The conclusion is drawn that the lipid particles in chyle depend for their stability on a phospholipid surface layer. The change which has been observed to occur in normal plasma is not easily related to physiological conditions.