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A DEMONSTRATION OF THE SECRETION OF PEPSIN BY THE ISOLATED FROG STOMACH
Author(s) -
Zwarenstein H.
Publication year - 1953
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1953.sp001032
Subject(s) - pepsin , q10 , stomach , chemistry , perforation , peptic , chromatography , medicine , biochemistry , biology , anatomy , peptic ulcer , materials science , enzyme , respiration , metallurgy , punching
1. When the isolated stomach of Xenopus lævis , ligatured at both ends, is filled with an acid solution, perforation of the stomach wall occurs near the cardiac end. This preparation was used to study the influence of pH and temperature on the action of pepsin in situ . 2. The optimum pH varies with the temperature. At 30° it is 0·45, at 40°, 1·0, and at 50° , 1·33. The pH range within which pepsin is active is 0·00‐3·75 at 30°, 0·05‐3·95 at 40°, and 0·39‐5·35 at 50°. 3. The Q10 is constant within a wide range of pH values. It decreases with a rise of temperature throughout the pH range of peptic activity.