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THE OXIDATION OF OXYHÆMOGLOBINS FROM DIFFERENT ANIMAL SPECIES
Author(s) -
Eggleton Philip,
Fegler George
Publication year - 1952
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1952.sp000985
Subject(s) - iodine , chemistry , hemoglobin , ferricyanide , biochemistry , organic chemistry
1. Hæmoglobin solutions derived from horse, ox, and dog blood were treated with graded amounts of ( a ) ferricyanide and ( b ) iodine, and the resulting methæmoglobin formation measured spectrophoto‐metrically. 2. It was found that when oxyhæmoglobin solutions are partially oxidized with iodine at pH 5·8 a considerable proportion of iodine oxidizes some part of the molecule other than the hæm. 3. When the iodine added is enough to produce 50 per cent. oxidation of the hæm, the globin has reduced 2 equivalents (horse), 4·5 equivalents (ox), and 6·5 equivalents (dog) of iodine respectively.