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INTRACORPUSCULAR METHÆMOGLOBIN FORMATION AND ITS RELATION TO THE RATE OF OXYGEN RELEASE
Author(s) -
Fegler G.
Publication year - 1948
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1948.sp000933
Subject(s) - chemistry , sodium azide , meth , hemoglobin , deoxygenation , azide , oxygen , sodium , biochemistry , organic chemistry , monomer , catalysis , polymer , acrylate
1. The relative rate of O 2 release from horse r.b.c. suspensions (pH 7·0–7·4) measured with a slow reaction method was investigated in relation to the methæmoglobin formation. 2. A straight‐line relationship was found between the relative rate of methæmoglobin formation by sodium nitrite and the increase in the rate of O 2 release. 3. A close relationship was also found between the increase in the rate of O 2 release and the relative rate of the spontaneous methæmoglobin formation caused by exposure of the r.b.c. suspensions to N 2 + CO 2 gas mixtures. 4. A methæmoglobin formation uninterrupted by the reduction develops in the suspensions poisoned by sodium azide. The phenomenon is probably caused by a combination between sodium azide and spontaneously formed methæmoglobin [Keilin], this compound being resistant to the intracorpuscular enzymatic reduction of the preformed methæmoglobin. Sodium azide also causes an increase in the rate of O 2 release from r.b.c. suspensions. 5. A suggestion is forwarded of a possible part played by the spontaneous methæmoglobin formation as a factor promoting deoxygenation.