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SPONTANEOUS INTRACORPUSCULAR INACTIVATION AND REACTIVATION OF HÆMOGLOBIN
Author(s) -
Fegler G.
Publication year - 1948
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1948.sp000932
Subject(s) - deoxygenation , chemistry , hemoglobin , oxygen , intracellular , biophysics , in vivo , stereochemistry , biochemistry , biology , catalysis , organic chemistry , microbiology and biotechnology
1. The intracorpuscular inactivation of hæmoglobin (presumably methæmoglobin formation) occurred in the suspensions of the horse r.b.c. (pH 7·4–7·3) when these were exposed to gas mixtures of air + CO 2 , oxygen + CO 2 , and N 2 + CO 2 for different periods of time (2–15 minutes). Also inactivation occurred in the r.b.c. suspensions in buffer solutions of pH 7·2–7·1 when exposed to air or nitrogen. 2. The reactivation (reduction of methæmoglobin) succeeds the inactivation if the exposure to N 2 + CO 2 or N 2 lasts longer than several minutes. The phenomenon may be expressed by a U‐shaped curve showing the phases of inactivation, equilibrium, and reactivation. 3. The extent of reactivation depends on the degree of deoxygenation, while the intracellular decrease in pH is a main factor of the inactivation. 4. Temperatures in the region of 38°C. favour the inactivation as well as the reactivation. 5. Attempts were made to imitate in vitro the changing conditions to which the blood is exposed in the vascular bed by subjecting the suspensions to suitable changes of gas phase. 6. The results obtained were discussed in relation to in vivo conditions.