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AN APPLICATION OF BONE‐MARROW CULTURES TO TOXICOLOGY AND THERAPEUTICS
Author(s) -
Harrison K.,
Randoll F. W.
Publication year - 1948
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1948.sp000921
Subject(s) - bone marrow , toxicity , chemistry , phenols , pharmacology , antidote , toxicology , medicine , biochemistry , organic chemistry , pathology , biology
1. A method of cultivating bone marrow in vitro is described, together with its applications to toxicology and therapeutics. 2. BAL and BAL‐Intrav have been compared as antidotes to arsenicals. BAL‐Intrav is less toxic than BAL, but is less potent as an antidote to lewisite oxide, and the range of effective therapeutic concentrations is smaller. The reaction product of BAL and Mapharside is toxic; the toxicity is diminished by an excess of BAL. The reaction product between BAL‐Intrav and Mapharside appears to be less toxic. 3. Extending the comparison to mercury and antimony, it appears that BAL‐Intrav is superior as an antidote to BAL. Both substances form toxic reaction products with antimony potassium tartrate and with Neostam; it is suggested that in the intact animal every effort should be made to encourage the excretion of these products. 4. Benzene is not toxic to bone marrow; of its oxidation products, the dihydric phenols are much more toxic than the trihydric phenols or than phenol itself. We are indebted to Surgeon‐Lieut. N. M. Hancox, R.N.V.R., for allowing us to make use of his work on the culture of bone marrow; to Mr. P. D. Mitchell, Dr. L. N. Owen, and Dr. L. A. Stocken for gifts of chemicals; to Mr. R. M. Weston, F.R.P.S., for the photographs; to Professor R. A. Peters, F.R.S., Professor G. R. Cameron, F.R.S., and Surgeon‐Captain A. Fairley, R.N., for advice and criticism; and to the Chief Scientist, Ministry of Supply, for permission to publish this paper, the substance of which has already appeared in Porton Reports (1944‐45).