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A STUDY OF SOME CONDITIONS INFLUENCING THE RATE OF EXCHANGE OF OXYGEN IN BLOOD IN VITRO
Author(s) -
Fegler J.,
Banister Jean
Publication year - 1946
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1946.sp000905
Subject(s) - oxygen , chemistry , carbon dioxide , volume (thermodynamics) , red blood cell , viscosity , partial pressure , analytical chemistry (journal) , chromatography , thermodynamics , biochemistry , organic chemistry , physics
A tonometer is illustrated which ensures constant conditions of exposure of blood to gas mixtures for any desired interval of time, making possible a simultaneous measurement of the velocity of exchange of oxygen and carbon dioxide in whole blood. The influence of variations in temperature, red‐cell volume p. c., the relative viscosity of the blood, and in the pressure gradient of oxygen between the blood and the gas phase on the rate of exchange of oxygen by oxalated blood in vitro was investigated. It was found that 1. An increase of temperature, or a reduction in the relative viscosity of the blood or in its relative red‐cell volume augmented the rates of uptake and release of oxygen. 2. The increases in the rate of exchange observed with rises of temperature from 20–38°C., or with reductions in the relative red‐cell volume corresponded to just such increases in the rate of exchange as would be expected from the resulting alterations in the relative viscosity of the blood. 3. The rate of oxygen uptake varied in almost direct proportion with the partial pressure of oxygen to which the blood was exposed. The rate of oxygen release did not so accurately reflect the pressure gradient of oxygen between the blood and the gas phase.