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INTERCELLULAR PLASMA IN THE CENTRIFUGED ERYTHROCYTES OF NORMAL HUMAN BLOOD
Author(s) -
Maizels Montague
Publication year - 1945
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1945.sp000900
Subject(s) - buffy coat , intracellular , chromatography , chemistry , significant difference , biology , biochemistry , immunology , medicine
If blood is centrifuged in tubes of 7 mm. bore, the intercellular plasma amounts to 2·7 per cent. of packed cells if the buffy coat is retained and to about 2·25 per cent. if the buffy coat is removed. In narrower tubes the values are slightly less. This intercellular plasma accounts for the difference observed between the true cell volume and the volume observed on centrifuging. The difference is so small that it may be neglected for practical purposes.