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CITRIC ACID‐SODIUM CITRATE‐GLUCOSE MIXTURES FOR BLOOD STORAGE
Author(s) -
Loutit J. F.,
Mollison P. L.,
Young I. Maureen,
Lucas E. J.
Publication year - 1943
Publication title -
quarterly journal of experimental physiology and cognate medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0033-5541
DOI - 10.1113/expphysiol.1943.sp000882
Subject(s) - trisodium citrate , citric acid , preservative , sodium citrate , chemistry , sodium , in vivo , potassium , biochemistry , medicine , food science , biology , microbiology and biotechnology , organic chemistry , pathology
1. Certain citric acid‐sodium citrate‐glucose mixtures have been tested and found to be satisfactory for use as blood preservatives. The use of these solutions is recommended for the following reasons:— ( a ) Red cells stored in these solutions survive better in the recipient's circulation after transfusion than when stored in any other solution yet tested. ( b ) The whole mixtures can be autoclaved with the production of little or no caramel. ( c ) The transfusion of blood stored in these solutions has not been accompanied by the production of any untoward results. ( d ) The amount of methæmoglobin formed when blood is stored with these solutions is not significantly greater than the amount formed when the usual trisodium citrate‐glucose solution is used. 2. The rates of release of inorganic phosphorus, of potassium shift, of glycolysis, of hæmolysis and of alteration in the osmotic fragility of blood stored in these solutions and in the Rous‐Turner and standard M. R. C. citrate‐glucose solutions were investigated. The changes observed were not found to be correlated with the ability of the erythrocytes to survive In Vivo after transfusion, except that in this series delay in hæmolysis was associated with improved survival in vivo. We should like to thank Mr. R. J. Bromfield, Dr. C. H. Gray, and Miss M. Heppenstall for carrying out some of the tests in this investigation; Dr. M. Maizels and Dr. M. M. Murray for advice; the Medical Superintendents of Kingston County and St. Helier Hospitals for access to suitable cases; and British Drug Houses Ltd. for supplies of reagents.