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THE MOUSE‐UNIT OF ANTERIOR PITUITARY HORMONE B
Author(s) -
Kennedy Walter P.
Publication year - 1933
Publication title -
quarterly journal of experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0370-2901
DOI - 10.1113/expphysiol.1933.sp000610
Subject(s) - anterior pituitary , limiting , endocrinology , medicine , pituitary gland , growth hormone , hormone , biology , chemistry , mechanical engineering , engineering
A description is given of a method of bio‐assay of the luteinising, or B hormone, of the anterior pituitary gland. The effects of partition of the dose are discussed. It is shown that the amount required to produce the chosen limiting reaction is ten times greater when given in one dose than when split into six parts. The differential production of hæmorrhagic follicles and of corpora lutea does not depend on dosage. Uterine growth follows the administration of “Prolan,” but quantitative measurements show the degree of growth to be irregular. The mouse‐unit of anterior pituitary hormone B (prolan B) is defined as the amount which, divided into six doses and injected intraperitoneally into 10 female mice exactly three weeks old, over a period of 36 hours, will produce hæmorrhagic follicles in 50 per cent. of the animals 100 hours from the first administration. The bulk of this investigation was carried out during my tenure of a Beit Memorial Research Fellowship. The cost of the experiments has been borne by a grant from the Ella Sachs Plotz Foundation. Bayer's Products, Ltd., kindly supplied the “Prolan” used.