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STUDIES ON THE ACCELERATION AND INHIBITION OF HÆMOLYSIS. IV. THE EFFECT OF INITIAL pH ON SAPONIN, TAUROCHOLATE, AND GLYCOCHOLATE HÆMOLYSIS
Author(s) -
Gordon Albert S.
Publication year - 1933
Publication title -
quarterly journal of experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0370-2901
DOI - 10.1113/expphysiol.1933.sp000587
Subject(s) - chemistry , sodium , saponin , lysis , chromatography , alkali metal , hydrochloric acid , bile acid , biochemistry , inorganic chemistry , organic chemistry , medicine , alternative medicine , pathology
1. It is shown that hæmolytic systems containing saponin, sodium taurocholate, or sodium glycocholate as the lysin are affected by initial pH in the following manner:— (a) The rendering of systems more acid than pH 6•0 results in an acceleration which increases with the concentration of acid employed except in the case of lysis produced by high concentrations of sodium taurocholate, where the addition of acid results in an increasing inhibition up to pH 4•5, beyond which the addition of more acid results in acceleration. (b) Raising the pH beyond 6•0 results in an inhibition which reaches its maximum in the case of the bile salts at about pH 8•0, and in the case of saponin between pH 9•0 and pH 10•0. (c) High concentrations of acid as well as alkali cause acceleration in all the systems. 2. Although the red cell is directly affected by high concentrations of acid or alkali, in moderate concentrations the effect is primarily upon the lysin. 3. The empirical expression which has been employed to describe certain well‐known cases of inhibition is found to be applicable to the types of inhibition and acceleration encountered in this study.