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STUDIES ON THE ACCELERATION AND INHIBITION OF HÆMOLYSIS. III. THE EFFECT OF MONOVALENT CATIONS ON SAPONIN HÆMOLYSIS
Author(s) -
Gordon Albert S.
Publication year - 1933
Publication title -
quarterly journal of experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0370-2901
DOI - 10.1113/expphysiol.1933.sp000586
Subject(s) - chemistry , haemolysis , electrolyte , rubidium , potassium , dilution , antagonism , inorganic chemistry , sodium , biochemistry , organic chemistry , biology , physics , receptor , electrode , immunology , thermodynamics
1. The effects of monovalent cations in hæmolytic systems, in which saponin is the lysin, depend not only on the dilution of lysin and type of red cell employed, but also upon the concentration of electrolyte present. 2. Introduction of potassium, lithium, or rubidium into systems containing human erythrocytes results in an acceleration of hæmolysis, whereas the addition of cæsium causes an inhibition. This is true only when sodium chloride is being replaced from the standard system. 3. Similar results are not obtained when ox cells are employed. Here certain concentrations of electrolyte produce acceleration while others cause inhibition. 4. A “polar antagonism” exists between cæsium and rubidium in systems containing human cells. 5. The various time‐dilution curves corresponding to the addition of different amounts of electrolyte are excellently described by the equation of Ponder and Yeager .