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THE RELATION OF PROTEINS TO CRYSTALLOIDS. III. HÆMOLYSIS BY ALKALI. IV. HÆMOLYSIS BY HYPOTONIC SODIUM CHLORIDE SOLUTIONS. V. HÆMOLYSIS BY RISE OF TEMPERATURE
Author(s) -
Roaf H. E.
Publication year - 1912
Publication title -
quarterly journal of experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0370-2901
DOI - 10.1113/expphysiol.1912.sp000110
Subject(s) - chemistry , dilution , sodium , tonicity , osmotic pressure , alkali metal , chlorine , chloride , osmosis , chromatography , inorganic chemistry , membrane , thermodynamics , biochemistry , organic chemistry , physics
1. Addition of alkali to sodium chloride causes an increase in the osmotic pressure of a hæmoglobin solution in equilibrium with it. 2. This increase of pressure runs parallel to the hæmolytic action of the alkalies. 3. Therefore hæmolysis by alkalies may be due to an increase in the osmotic pressure of the hæmoglobin contained in the corpuscles. 4. The experiments failed to show that hæmolysis by acid is accompanied by an increase in the osmotic pressure of the hæmoglobin, but such a possibility is discussed. 5. Hæmolysis by dilution is probably the result of a temporary difference of osmotic pressure. (a) If the corpuscle wall is impermeable to sodium and chlorine, a difference in pressure of over 1000 mm. of mercury exists before hæmolysis occurs. (b) The osmotic pressure of hæmoglobin does not show an increase corresponding to hæmolysis by dilution. 6. Hæmolysis by rise of temperature may be due to the increase in the osmotic pressure of hæmoglobin. 7. The relation of cells to inorganic substances can be explained without the assumption that the cell membrane will not allow these substances to pass through.