Premium
Effect of Polyphenolic Compounds on the Renal Na + ,K + ‐ATPase during the Restoration of Normotension after Experimentally Induced Hypertension in Rats
Author(s) -
Javorková Veronika,
Pechánová Olga,
Andriantsitohaina Ramaroson,
Vrbjar Norbert
Publication year - 2003
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/eph8802553
Subject(s) - chemistry , polyphenol , nitric oxide , atpase , nitric oxide synthase , kinetics , enzyme , medicine , endocrinology , biophysics , biochemistry , biology , organic chemistry , antioxidant , physics , quantum mechanics
It is commonly known that consumption of foods and beverages rich in polyphenols is associated with a lower incidence of cardiovascular disease. The purpose of this study was to assess whether the application of red wine polyphenols influences the kinetic properties of renal Na + ,K + ‐ATPase in rats in which hypertension has been experimentally induced by the nitric oxide synthase inhibitor L‐NAME. Treatment with polyphenols during the recovery from hypertension to normotension resulted in the complete revival of the functional properties of the Na + ,K + ‐ATPase, as indicated by the total restoration of K m , K Na (concentration of Na + necessary to achieve half‐maximal reaction velocity) and V max for enzyme activation by ATP and/or Na + to pre‐hypertension values. Two positive effects of polyphenols during the recovery period are indicated: a restoration of the affinity of the ATP and Na + binding sites to control values and a probable increase in the number of Na + ,K + ‐ATPase molecules to a level comparable to that in control conditions, as suggested by the complete renewal of V max .