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Interactions Between Neural and Hormonal Mediators of Renal Vascular Tone in Anaesthetized Rabbits
Author(s) -
Guild SarahJane,
Barrett Carolyn J.,
Evans Roger G.,
Malpas Simon C.
Publication year - 2003
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/eph8802462
Subject(s) - renal blood flow , medicine , vasopressin , endocrinology , angiotensin ii , vasodilation , kidney , perfusion , vasoactive intestinal peptide , renal circulation , blood pressure , neuropeptide , receptor
We investigated how sympathetic nerve activity and vasoactive hormones interact in controlling renal haemodynamics in pentobarbitone‐anaesthetized rabbits. Renal blood flow was progressively reduced by electrical stimulation (0.5‐3 Hz) of the renal nerves, during renal arterial infusion of saline, vasoconstrictors (angiotensin II and [Phe 2 ,Ile 3 ,Orn 8 ]‐vasopressin), or vasodilators (acetylcholine, adrenomedullin and the nitric oxide donor methylamine hexamethylene NONOate (MAHMA NONOate). A frequency‐rich stimulus was also applied to test whether the vasoactive agents affect the dynamic control of renal blood flow by sympathetic nerve activity. The vasodilators tended to increase renal blood flow, but only the effect of MAHMA NONOate was statistically significant. [Phe 2 ,Ile 3 ,Orn 8 ]‐vasopressin reduced medullary perfusion (by 61 ± 12%) but not renal blood flow or cortical perfusion. Angiotensin II reduced renal blood flow (33 ± 3%) and cortical perfusion (14 ± 5%) but not medullary perfusion. Steady‐state responses of renal blood flow and cortical perfusion during renal nerve stimulation were attenuated during infusion of acetylcholine and [Phe 2 ,Ile 3 ,Orn 8 ]‐vasopressin, while angiotensin II attenuated responses of medullary perfusion, and MAHMA NONOate and adrenomedullin had no significant effects. The dynamic response to sympathetic nerve activity (renal blood flow responded as a low pass filter with a pure time delay of ∼664 ms) was not altered by the vasoactive agents. We conclude that some vasoactive agents can modulate steady‐state renal haemodynamic responses to sympathetic nerve activity in a regionally specific manner, independent of their effects on baseline renovascular tone. However, they have little impact on the dynamic response of renal blood flow to sympathetic nerve activity.