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Overexpression of NDRG2 in skeletal muscle does not ameliorate the effects of stress in vivo
Author(s) -
Mir Bilal A.,
Mason Shaun A.,
May Anthony K.,
Russell Aaron P.,
Foletta Victoria C.
Publication year - 2020
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/ep088620
Subject(s) - skeletal muscle , endogeny , endocrinology , unfolded protein response , medicine , oxidative stress , amyotrophic lateral sclerosis , tibialis anterior muscle , endoplasmic reticulum , sod1 , mitochondrion , genetically modified mouse , biology , transgene , chemistry , microbiology and biotechnology , disease , gene , biochemistry
New FindingsWhat is the central question of this study? Do elevated levels of the stress‐response protein NDRG2 protect against fasting and chronic disease in mouse skeletal muscle?What is the main finding and its importance? NDRG2 levels increased in the tibialis anterior muscle in response to fasting and the effects of motor neurone disease. No alleviation of the stress‐related and proteasomal pathways, mitochondrial dysfunction or muscle mass loss was observed even with the addition of exogenous NDRG2 indicating that the increase in NDRG2 is a normal adaptive response.Abstract Skeletal muscle mass loss and dysfunction can arise from stress, which leads to enhanced protein degradation and metabolic impairment. The expression of N‐myc downstream‐regulated gene 2 (NDRG2) is induced in response to different stressors and is protective against the effects of stress in some tissues and cell types. Here, we investigated the endogenous NDRG2 response to the stress of fasting and chronic disease in mice and whether exogenous NDRG2 overexpression through adeno‐associated viral (AAV) treatment ameliorated the response of skeletal muscle to these conditions. Endogenous levels of NDRG2 increased in the tibialis anterior muscle in response to 24 h fasting and with the development of the motor neurone disease, amyotrophic lateral sclerosis, in SOD1 G93A transgenic mice. Despite AAV‐induced overexpression and increased expression with fasting, NDRG2 was unable to protect against the activation of proteasomal and stress pathways in response to fasting. Furthermore, NDRG2 was unable to reduce muscle mass loss, mitochondrial dysfunction and elevated oxidative and endoplasmic reticulum stress levels in SOD1 G93A mice. Conversely, elevated NDRG2 levels did not exacerbate these stress responses. Overall, increasing NDRG2 levels might not be a useful therapeutic strategy to alleviate stress‐related disease pathologies in skeletal muscle.