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A 10 km time trial running bout acutely increases the number of angiogenic T cells in the peripheral blood compartment of healthy males
Author(s) -
Ross Mark,
Tormey Peter,
Ingram Lesley,
Simpson Richard,
Malone Eva,
FloridaJames Geraint
Publication year - 2016
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/ep085771
Subject(s) - medicine , flow cytometry , cd31 , cd8 , peripheral blood mononuclear cell , stromal cell , immunology , endocrinology , angiogenesis , biology , in vitro , immune system , biochemistry
New FindingsWhat is the central question of the study? Are CD31 + angiogenic T (T ANG ) cells preferentially mobilized in response to acute exercise?What is the main finding and its importance? Our study reveals that T ANG cells are redistributed into the circulation in response to acute strenuous exercise, but to a lesser extent than CD31 − T cells. Of the T ANG cells mobilized, T ANG cells expressing CXCR4 show greater redistribution compared with CXCR4 − T ANG cells. Stromal‐derived factor 1‐α does not appear to play a role in the redistribution of T ANG cells expressing CXCR4. The results suggest that a single bout of strenuous exercise might provide a short vasculogenic window, which could benefit the vascular system by redistributing CD31 + T ANG cells.CD31 + T cells have been documented to possess vasculogenic properties and have been termed ‘angiogenic T cells’ (T ANG cells). No study to date has fully characterized the effect of acute exercise on T ANG cells. Twelve male participants aged 24–45 years undertook a running 10 km time trial, with peripheral blood samples taken before, immediately after and 1 h postexercise for quantification of T ANG cells and subsequent CXCR4 cell surface expression by flow cytometry. The T ANG cells demonstrated a 102% increase in number in the peripheral circulation immediately postexercise compared with pre‐exercise levels, followed by a large egress (50%) from the circulation in total T ANG cells 1 h postexercise. This was due to changes in both CD4 + and CD8 + T ANG cells, with CD8 + T ANG cells displaying greater ingress (123%) and egress (52%) compared with CD4 + T ANG cells (ingress, 83%; egress, 37%). The cell surface expression intensity of CXCR4 was affected only on CD8 + T ANG cells, with a significant increase in cell surface expression immediately postexercise versus pre‐exercise levels. The CD31 − T cells displayed greater redistribution than CD31 + T ANG cells (119 versus 102%). CXCR4‐expressing T ANG cells showed greater response to acute exercise than CXCR4 − cells, which was accompanied by large changes in CXCR4 ligand SDF‐1α. The results show that acute exercise increases T ANG cells in the circulation in response to an acute exercise stressor. Additionally, CXCR4 cell surface expression appears to be increased in response to exercise, which may result from the direct upregulation of CXCR4 on the T‐cell surface or could be due to CD31 + T cells being redistributed into the blood expressing greater levels of CXCR4.