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Acute bout of resistance exercise increases vitamin D receptor protein expression in rat skeletal muscle
Author(s) -
Makanae Yuhei,
Ogasawara Riki,
Sato Koji,
Takamura Yusuke,
Matsutani Kenji,
Kido Kohei,
Shiozawa Naruhiro,
Nakazato Koichi,
Fujita Satoshi
Publication year - 2015
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1113/ep085207
Subject(s) - calcitriol receptor , medicine , endocrinology , skeletal muscle , endurance training , vitamin d and neurology , stimulation , receptor , vitamin
New FindingsWhat is the central question of this study? Does an acute bout of exercise alter vitamin D receptor expression in rat skeletal muscle?What is the main finding and its importance? Resistance exercise but not endurance exercise increased intramuscular vitamin D receptor expression. Thus, resistance exercise may be an effective way to increase muscle vitamin D receptor expression.Vitamin D and vitamin D receptor (VDR) are involved in the maintenance of skeletal muscle mass and function. Although resistance exercise is well known to enhance muscle growth and improve muscle function, the effect of resistance exercise on VDR has been unclear. We investigated intramuscular VDR expression in response to an acute bout of resistance exercise or endurance exercise. Male adult Sprague–Dawley rats were subjected to either resistance exercise (isometrically exercised via percutaneous electrical stimulation for five sets of ten 3 s contractions, with a 7 s interval between contractions and 3 min rest intervals between sets) or endurance exercise (treadmill at 25 m min −1 for 60 min). Rats were killed immediately or 1, 3, 6 or 24 h after completion of the resistance or endurance exercise, and gastrocnemius muscles were removed. Non‐exercised control animals were killed in a basal state (control group). Intramuscular VDR expression was significantly higher immediately after resistance exercise and elevated for 3 h after exercise compared with the control group ( P < 0.05), and the resistance exercise significantly increased phosphorylated ERK1/2 and Mnk1 expression ( P < 0.05), which may be associated with VDR expression, immediately after exercise. Additionally, intramuscular expression of cytochrome P450 27B1, an enzyme related to vitamin D metabolism, was significantly higher at 1 and 3 h after exercise ( P < 0.05) compared with the control group. In contrast, endurance exercise had no effect on any of the measured proteins. Our results indicate that resistance exercise may be an efficient way to increase intramuscular VDR and related enzyme expression.