Open AccessANTI-QUORUM SENSING AND ANTIBIOTIC ENHANCEMENT OF ALLYLPYROCATECHOL AND METHYL GALLATE
Author(s) -
Carine S. S. Lim,
Eric Wei Chiang Chan,
Chen Wai Wong,
Joash Ban Lee Tan,
VimalaDewi Anggraeni,
Z. J. Loong,
Yong Kiat Teo
Publication year - 2021
Publication title -
jurnal teknologi/jurnal teknologi
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.191
H-Index - 22
eISSN - 2180-3722
pISSN - 0127-9696
DOI - 10.11113/jurnalteknologi.v83.15146
Subject(s) - chromobacterium violaceum , quorum sensing , chemistry , pseudomonas aeruginosa , betel , staphylococcus aureus , antibiotics , traditional medicine , microbiology and biotechnology , streptomyces coelicolor , antimicrobial , bacteria , biology , biochemistry , organic chemistry , medicine , nut , mutant , engineering , gene , genetics , structural engineering , virulence
In this study, the antibiotic enhancement and quorum sensing (QS) inhibition of allylpyrocatechol (APC) and methyl gallate (MG) isolated from leaves of Piper betle (betel) and leaf shoots of Anacardium occidentale (cashew), respectively, were compared. When applied with streptomycin and tested against Staphylococcus aureus and Pseudomonas aeruginosa, the effect of APC was additive while that of MG was synergistic. In terms of QS inhibition against Chromobacterium violaceum, both compounds were equally effective with violacein inhibition observed at concentrations of 60 μg/mL. We successfully increased the purity of APC in the methanol betel leaf extract from 13.2% to 31.5% w/w through solvent partitioning. This study is the first to document the anti-QS activity of APC, which is comparable to that of MG.