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C ryptosporidium hominis Subtypes and E nterocytozoon bieneusi Genotypes in HIV ‐Infected Persons in I badan, N igeria
Author(s) -
Ayinmode A. B.,
Zhang H.,
DadaAdegbola H. O.,
Xiao L.
Publication year - 2014
Publication title -
zoonoses and public health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.87
H-Index - 65
eISSN - 1863-2378
pISSN - 1863-1959
DOI - 10.1111/zph.12072
Subject(s) - enterocytozoon bieneusi , microsporidiosis , genotype , cryptosporidium , biology , virology , polymerase chain reaction , internal transcribed spacer , feces , nested polymerase chain reaction , microbiology and biotechnology , gene , ribosomal rna , genetics , microsporidia , spore
Summary C ryptosporidium and E nterocytozoon are common opportunistic pathogens in HIV + patients in developing countries, especially those do not have access to antiretroviral therapy. To determine the distribution of genotypes/subtypes of C ryptosporidium and E nterocytozoon bieneusi , faecal specimens were collected from 132 HIV + persons attending a tertiary hospital in I badan, N igeria. By polymerase chain reaction, eight and ten patients were identified as positive for C ryptosporidium spp. and E . bieneusi, respectively. Seven of the C ryptosporidium specimens were identified as C . hominis, while the remaining one as the new species C . viatorum recently identified in the U nited K ingdom. DNA sequencing of the 60‐kDa glycoprotein gene showed that the C . hominis belonged to three common subtype families: Ia (in three patients), Ib (in one patient) and Ie (in one patient). In contrast, DNA sequencing of the E . bieneusi internal transcribed spacer products showed the occurrence of genotypes associated with both humans (Peru 8 in one patient, Nig2 in two patients and a new genotype in one patient) and animals (D in one patient and Type IV in five patients). Low CD 4+ cell count was identified as a risk factor for both cryptosporidiosis and microsporidiosis.