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Riboflavin photo‐cross‐linking method for improving elastin stability and reducing calcification in bioprosthetic heart valves
Author(s) -
Lei Yang,
Guo Gaoyang,
Jin Wanyu,
Liu Mengwei,
Wang Yunbing
Publication year - 2019
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/xen.12481
Subject(s) - elastin , calcification , riboflavin , chemistry , collagenase , glutaraldehyde , elastase , in vivo , biochemistry , enzyme , pathology , medicine , biology , chromatography , microbiology and biotechnology
Abstract Background Glutaraldehyde cross‐linked bioprosthetic heart valves might fail due to progressive degradation and calcification. Methods In this study, we developed a new BHVs preparation strategy named as “HPA/TRA/FMN” that utilized 3,4‐hydroxyphenylpropionic acid (HPA)/tyramine (TRA) conjugated pericardium and riboflavin 5’‐monophosphate (FMN) initiated photo‐cross‐linking method. HPA/TRA‐pericardium conjugation would provide extra phenol groups for FMN initiated photo‐cross‐linking. Results The feeding ratio of riboflavin 5’‐monophosphate was optimized. The collagenase and elastase enzymatic degradation in vitro, biomechanics, calcification, elastin stability in vivo, and macrophage marker CD68 were characterized. We demonstrated that riboflavin photo‐cross‐linked pericardiums had great collagen and elastin stability, improved mechanical properties, better resistance for calcification, and less CD68 positive macrophages in rat subdermal implantation study. Conclusions This new riboflavin photo‐cross‐linking strategy would be a promising method to make BHVs which have better elastin stability, less calcification, and reduced inflammatory response.