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Effect of an oxygen‐generating scaffold on the viability and insulin secretion function of porcine neonatal pancreatic cell clusters
Author(s) -
Lee Eun Mi,
Jung JiIn,
Alam Zahid,
Yi HeeGyeong,
Kim Heejin,
Choi Jin Woo,
Hurh Sunghoon,
Kim Young June,
Jeong Jong Cheol,
Yang Jaeseok,
Oh KookHwan,
Kim Hee Chan,
Lee Byeong Chun,
Choi Inho,
Cho DongWoo,
Ahn Curie
Publication year - 2018
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/xen.12378
Subject(s) - islet , viability assay , chemistry , scaffold , reactive oxygen species , oxygen , cell , insulin , microbiology and biotechnology , biochemistry , biomedical engineering , biology , medicine , organic chemistry
Background Islet encapsulation techniques have shown limited success in maintaining islet survival and function because encapsulation decreases oxygen supply. In this study, an oxygen‐generating scaffold was fabricated to prevent hypoxic cell damage and improve the viability and insulin secretion of islets. Methods We fabricated an oxygen‐generating scaffold by mixing calcium peroxide (CaO 2 ) with polydimethylsiloxane ( PDMS ). We evaluated the effects of the oxygen‐generating PDMS + CaO 2 scaffold on viability, caspase‐3 and caspase‐7 activity, oxygen consumption rate ( OCR ), glucose‐stimulated insulin secretion ( GSIS ), hypoxic cell marker expression, and reactive oxygen species ( ROS ) levels in porcine neonatal pancreatic cell clusters ( NPCC s). We also fabricated a microfluidic device that allowed measuring the effects of the oxygen‐generating scaffold on viability. Results Oxygen generation by the PDMS + CaO 2 scaffold was sustained for more than 24 hours in vitro. NPCC s encapsulated in PDMS + CaO 2 showed higher viability than NPCC s in PDMS scaffolds and control NPCC s grown without a scaffold. PDMS + CaO 2 ‐encapsulated NPCC s showed lower caspase‐3 and caspase‐7 activity, hypoxic cell expression, and ROS levels, and higher OCR and GSIS than those in PDMS or control cells. Using the microfluidic device, we observed that the viability of PDMS + CaO 2 ‐encapsulated NPCC s was higher than that of PDMS ‐encapsulated NPCC s. Conclusions NPCC s in PDMS + CaO 2 scaffolds show higher viability and insulin secretion than do NPCC s in PDMS scaffolds and control cells. Therefore, this oxygen‐generating scaffold has potential for application in future islet transplantation studies.