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Magnetic separation of encapsulated islet cells labeled with superparamagnetic iron oxide nano particles
Author(s) -
Mettler Esther,
Trenkler Anja,
Feilen Peter J.,
Wiegand Frederik,
Fottner Christian,
Ehrhart Friederike,
Zimmermann Heiko,
Hwang Yong Hwa,
Lee Dong Yun,
Fischer Stefan,
Schreiber Laura M.,
Weber Matthias M.
Publication year - 2013
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/xen.12042
Subject(s) - islet , transplantation , islet cell transplantation , dextran , in vivo , chemistry , biophysics , diabetes mellitus , chromatography , medicine , endocrinology , biology , microbiology and biotechnology
Islet cell transplantation is a promising option for the restoration of normal glucose homeostasis in patients with type 1 diabetes. Because graft volume is a crucial issue in islet transplantations for patients with diabetes, we evaluated a new method for increasing functional tissue yield in xenogeneic grafts of encapsulated islets. Islets were labeled with three different superparamagnetic iron oxide nano particles ( SPIO Ns; dextran‐coated SPION , siloxane‐coated SPION , and heparin‐coated SPIO N). Magnetic separation was performed to separate encapsulated islets from the empty capsules, and cell viability and function were tested. Islets labeled with 1000 μg Fe/ml dextran‐coated SPIONs experienced a 69.9% reduction in graft volume, with a 33.2% loss of islet‐containing capsules. Islets labeled with 100 μg Fe/ml heparin‐coated SPIO Ns showed a 46.4% reduction in graft volume, with a 4.5% loss of capsules containing islets. No purification could be achieved using siloxane‐coated SPIO Ns due to its toxicity to the primary islets. SPION labeling of islets is useful for transplant purification during islet separation as well as in vivo imaging after transplantation. Furthermore, purification of encapsulated islets can also reduce the volume of the encapsulated islets without impairing their function by removing empty capsules.