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Human adipose‐derived mesenchymal stem cells can survive and integrate into the adult rat eye following xenotransplantation
Author(s) -
HaddadMashadrizeh Aliakbar,
Bahrami Ahmad R.,
Matin Maryam M.,
Edalatmanesh Mohammad A.,
Zomorodipour Alireza,
Gardaneh Mossa,
Farshchian Moein,
MomeniMoghaddam Madjid
Publication year - 2013
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1111/xen.12033
Subject(s) - mesenchymal stem cell , xenotransplantation , transplantation , stem cell , adipose tissue , regenerative medicine , microbiology and biotechnology , biology , immunology , adult stem cell , medicine , in vitro , endothelial stem cell , surgery , endocrinology , genetics
Background Novel threads of discovery provide the basis for optimism for the development of a stem‐cell‐based strategy for the treatment of retinal blindness. Accordingly, achievement to suitable cell source with potential‐to‐long‐term survival and appropriate differentiation can be an effective step in this direction. Methods After derivation of human adipose‐derived mesenchymal stem cells ( HAD ‐ MSC s), they were stably transfected with a vector containing Turbo‐green fluorescent protein ( GFP ) and JR ed to be able to trace them after transplantation. Labeled HAD ‐ MSC s were transplanted into the intact adult rat eye and their survival, integration, and migration during 6 months post‐transplantation were assessed. Results The transplanted cells were traceable in the rat vitreous humor ( VH ) up until 90 days after transplantation, with gradual reduction in numbers, their adhesion and expansion capacity after recovery. These cells were also integrated into the ocular tissues. Nonetheless, some of the implanted cells succeeded to cross the blood–retina barrier ( BRB ) and accumulate in the spleen with time. Conclusions The survival of the HAD ‐ MSC s for a period of 90 days in VH and even longer period of up to 6 months in other eye tissues makes them a promising source to be considered in regenerative medicine of eye diseases. However, the potency of crossing the BRB by the implanted cells suggests that use of HAD ‐ MSC s must be handled with extreme caution.