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Akermanite bioceramic enhances wound healing with accelerated reepithelialization by promoting proliferation, migration, and stemness of epidermal cells
Author(s) -
Wang Fangfang,
Wang Xiaoya,
Ma Kui,
Zhang Cuiping,
Chang Jiang,
Fu Xiaobing
Publication year - 2019
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12742
Subject(s) - wound healing , wnt signaling pathway , stem cell , cell growth , microbiology and biotechnology , epidermal growth factor , cancer research , chemistry , medicine , biology , immunology , signal transduction , biochemistry , receptor
ABSTRACT Reepithelialization is an important step of wound healing, which is mainly completed by proliferation and migration of epidermal cells. Akermanite is a Ca‐, Mg‐, and Si‐containing bioceramic. This study evaluated the effects of Akermanite on wound healing and investigated the mechanisms. Using scald burn mice models, we demonstrated that local Akermanite treatment significantly accelerated wound healing by increasing reepithelialization and the stemness of epidermal cells. Epidermal cells were cultured in medium containing Akermanite extracts to explore the cellular mechanism of reepithelialization. Akermanite promoted the cell proliferation and migration, maintaining more cells in the S and G 2 /M phases of the cell cycle. An additional study showed that Akermanite enhanced the expressions of integrinβ1, Lgr4, Lgr5, and Lgr6, which are specific molecular markers of epidermal stem cells, accompanied by the activation of the Wnt/β‐catenin pathway. These results suggested that Akermanite accelerated reepithelialization by increasing the proliferation, migration, and stemness of epidermal cells in a manner related to the Wnt/β‐catenin pathway, which might contribute, at least partially, to accelerated wound healing by Akermanite therapy.