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Development of a large‐volume human‐derived adipose acellular allogenic flap by perfusion decellularization
Author(s) -
Giatsidis Giorgio,
Guyette Jacques P.,
Ott Harald C.,
Orgill Dennis P.
Publication year - 2018
Publication title -
wound repair and regeneration
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.847
H-Index - 109
eISSN - 1524-475X
pISSN - 1067-1927
DOI - 10.1111/wrr.12631
Subject(s) - decellularization , perfusion , adipose tissue , medicine , biomedical engineering , tissue engineering , cardiology
In reconstructive surgery, transfer of patients’ tissue (autologous flaps) is routinely used to repair large soft tissue defects caused by surgery, trauma, chronic diseases, or malformations; unfortunately, this strategy is not always possible and often creates a secondary defect in the donor site of the tissue. Tissue‐engineered synthetic flaps are currently unable to repair clinically‐relevant, large‐volume defects; allogenic flaps from cadaveric donors could provide a ready‐to‐use biological alternative if treated with methods to avoid the immune‐rejection of the donor's cells. Here, we describe the successful decellularization of a large (> 800 cc) human‐derived adipose flap through a perfusion apparatus; we demonstrate the complete removal of the immunogenic cellular components of the flap with the retention of its structural components and vascular network. Our aim is to obtain a universally compatible, off‐the‐shelf acellular allogenic flap that could be recellularized with cells from recipient patients to provide a tissue‐engineered allogenic/autologous alternative for reconstruction of large‐volume soft‐tissue defects.